Home » Products » EzWay Annexin V-FITC Apoptosis Detection Kit LABISKOMA

Sale! Product

EzWay Annexin V-FITC Apoptosis Detection Kit LABISKOMA

Category:
Description
EzWay™ Annexin V-FITC Apoptosis Kit can identify apoptosis at an earlier stage than DNA-based assays such as TUNEL. Annexin V is a calcium-dependant phospholipid binding protein that has a high affinity for phosphatidylserine (PS), a plasma membrane phsopholipid. In non-apoptotic cells, most PS are localized at the inner layer of the plasma membrane, but after inducing apoptosis, PS are exposed to the extracellular environment, and then Annexin V binds to PS. Cells that stain positive for Annexin V-FITC (green) and negative for PI are undergoing apoptosis. Cells that stain positive for both Annexin V-FITC (green) and PI (red) are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both Annexin V-FITC and PI are alive and not undergoing measurable apoptosis.
Specification
• Utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis
• Annexin V FITC binds to the membranes of apoptotic cells
• Detection by flow cytometry or fluorescence microscopy
Components
• Annexin V-FITC
• 5X Binding buffer
• Propidium Iodide (PI)