Catalogue No. EU2548
Alias 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit, 8-oxo-DG ELISA Kit, 8-hydroxyһ2 deoxyguanosine ELISA Kit
Detection Method Competitive ELISA, Coated with Antigen
Application 8-OHdG ELISA Kit allows for the in vitro quantitative determination of 8-OHdG concentrations in plasma, tissue homogenates and other biological fluids.
Size 96T
Range1.563-100ng/ml
Sensitivity < 0.938ng/ml
Species Universal
Standard Curve:
Storage 4 ℃ for 6 months
Recovery: Matrices listed below were spiked with certain level of 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|---|---|
| serum(n=5) | 95-103 | 100 |
| EDTA plasma(n=5) | 87-105 | 94 |
| heparin plasma(n=5) | 85-101 | 90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-103% | 85-95% | 88-105% | 88-102% |
| EDTA plasma(n=5) | 84-100% | 84-101% | 82-101% | 84-93% |
| heparin plasma(n=5) | 81-94% | 93-100% | 82-96% | 80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only